Molecular Diagnostics 2nd Edition Buckingham Test Bank
Test Bank for Molecular Diagnostics Fundamentals Methods and Clinical Applications 2nd Edition By Lela Buckingham, ISBN-10: 0803626770, ISBN-13: 9780803626775
Chapter 1: DNA
Multiple Choice
Identify the choice that best completes the statement or answers the question.
____ 1. DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond?
a. Aldehyde
b. Ester
c. Phosphodiester
d. Peptide
____ 2. Nucleotides in DNA consist of which of the following?
a. Nitrogen base, deoxyribose, and phosphate
b. Nitrogen base, ribose, and sulfur
c. Carbon base, ribose, and phosphate
d. Carbon base, glucose, and carboxyl
____ 3. Which of the five ribose carbons of the deoxyribonucleotide are involved in formation of the DNA chain?
a. 1’ and 5’
b. 2’ and 4’
c. 3’ and 4’
d. 3’ and 5’
____ 4. In a DNA molecule, one end has a free hydroxyl group and one end has a free ___________.
a. phosphate group
b. hydroxyl group
c. amino group
d. carboxyl group
____ 5. What type of bonds spontaneously form between two complementary strands of DNA?
a. Hydrogen bonds
b. Covalent bonds
c. Phosphodiester bonds
d. Polar covalent bonds
____ 6. In a double-stranded DNA molecule, base pairing between strands occurs between:
a. A purine and a purine
b. A pyrimidine and a pyrimidine
c. A purine and a pyrimidine
d. Any type of nucleotide base
____ 7. Which of the following is a purine?
a. Thymine
b. Cytosine
c. Adenine
d. Alanine
____ 8. Which DNA sequence is complementary to 5GATTCTCAAAGGACT3?
a. 5GATTCTCAAAGGACT3
b. 3GATTCTCAAAGGACT5
c. 3CTAAGAGTTTCCTGA5
d. 5CTAAGAGTTTCCTGA3
____ 9. The term used to describe the arrangement of the individual strands in the double-stranded DNA molecule is:
a. Parallel
b. Antiparallel
c. Tangential
d. Divergent
____ 10. The process of separating the two DNA strands into two single strands is called:
a. Denaturation
b. Bidirectional
c. Depolymerization
d. Synthesis
____ 11. DNA replication is:
a. Conservative
b. Semiconservative
c. Nonconservative
d. Dispersive
____ 12. DNA replication requires the presence of:
a. DNA template
b. Pyrophosphate
c. Amino acids
d. Messenger RNA
____ 13. DNA replication proceeds with the new daughter strand synthesized in the following orientation:
a. 5to 3
b. 3to 5
c. The daughter strand is not synthesized in any order.
d. Either 5to 3or 3 to 5
____ 14. In DNA replication, the leading strand is the strand that has which orientation?
a. 5to 3
b. 3to 5
c. Both strands are leading.
____ 15. The lagging DNA strand is synthesized discontinuously producing:
a. Kornberg fragments
b. Southern fragments
c. Okazaki fragments
d. Klenow fragments
____ 16. Which of the following does not play a role in DNA replication?
a. RNA primer
b. Deoxynucleotide
c. Stop codon
d. DNA polymerase
____ 17. In DNA replication, where does replication begin and end?
a. Origin of replication; end of the molecule
b. Promoter; termination site
c. Start codon; stop codon
d. Start codon, termination site
____ 18. Which of the following enzymes will untangle DNA?
a. Polymerase
b. Helicase
c. Kinase
d. Phosphatase
____ 19. Restriction endonucleases are enzymes that are produced by bacteria and __________.
a. degrade viral proteins
b. digest DNA
c. have no laboratory applications
d. degrade lipids
____ 20. Enzymes that recognize palindromic sequences of DNA, that are cut within the recognition sequence, that do not have methylating activity, and that are used frequently in the laboratory are which type of restriction enzymes?
a. Type I
b. Type II
c. Type III
d. Type IV
____ 21. Which of the following is a type II restriction enzyme recognition site?
a. GAATTC
b. GAATTG
c. GAAAAG
d. GATCAG
____ 22. An exonuclease will catalyze what type of reaction?
a. Polymerization of nucleotides into long strands
b. Separation of complementary DNA strands
c. Dissociation of phosphodiester bonds at the ends of DNA molecules
d. Formation of circular DNA molecules from linear ones
____ 23. What reactions are catalyzed by DNA methyltransferase?
a. Addition of methyl groups to nitrogen bases
b. Addition of methyl groups to ribose sugars
c. Addition of methyl groups to proteins
d. Removal of methyl groups from DNA
____ 24. The purpose of ligase is to:
a. Synthesize DNA
b. Cut DNA within the double helix
c. Chew DNA from the ends
d. Paste two ends of DNA together
____ 25. What is the fertility factor (F) observed by Hayes and Lederberg?
a. A chemical that encourages bacterial mating
b. A virus
c. A plasmid
d. A polysaccharide on the cell surface
____ 26. Avery, MacLeod, and McCarty identified the nature of Griffith’s transforming factor in an experiment where it was destroyed by what treatment?
a. Protease
b. Ribonuclease
c. Deoxyribonuclease
d. Heating
____ 27. The movement of DNA from one bacterium to another through the activity of bacteriophages is called:
a. Conjugation
b. Transformation
c. Transduction
d. Crossing over
____ 28. In sexual recombination, new combinations of genes are created by which of the following processes?
a. Transduction
b. Crossing over
c. Conjugation
d. Transformation
____ 29. Which of the following promote resistance to common antibiotics?
a. Helicases
b. R factors
c. Endonucleases
d. Methylases
____ 30. Recombination between a plasmid and the cell chromosome will result in:
a. Rapid degradation of the chromosome
b. Death of the cell
c. Immediate cellular replication
d. Stable integration of plasmid genes into the chromosome
Chapter 1: DNA
Answer Section
MULTIPLE CHOICE
1. ANS: C PTS: 1
2. ANS: A PTS: 1
3. ANS: D PTS: 1
4. ANS: A PTS: 1
5. ANS: A PTS: 1
6. ANS: C PTS: 1
7. ANS: C PTS: 1
8. ANS: C PTS: 1
9. ANS: B PTS: 1
10. ANS: A PTS: 1
11. ANS: B PTS: 1
12. ANS: A PTS: 1
13. ANS: A PTS: 1
14. ANS: B PTS: 1
15. ANS: C PTS: 1
16. ANS: C PTS: 1
17. ANS: A PTS: 1
18. ANS: B PTS: 1
19. ANS: B PTS: 1
20. ANS: B PTS: 1
21. ANS: A PTS: 1
22. ANS: C PTS: 1
23. ANS: A PTS: 1
24. ANS: D PTS: 1
25. ANS: C PTS: 1
26. ANS: C PTS: 1
27. ANS: C PTS: 1
28. ANS: B PTS: 1
29. ANS: B PTS: 1
30. ANS: D PTS: 1
Molecular Diagnostics 2nd Edition TEST BANK
